To help differentiate between vaccine interference and wild-type (true) infection, IDEXX Reference Laboratories has developed the Canine Distemper Virus (CDV) Quant RealPCR™ Test . This test provides a quantitative measure of the CDV viral load, which is typically much higher during active infection compared to the level detected due to recent vaccination. It has been incorporated into IDEXX’s existing canine respiratory panel at no additional cost. A stand-alone test is also available for quantitative distemper virus information from swabs collected from respiratory mucosa, preferably deep pharyngeal. Results are available in 1-3 days.
In a screen for mutations of the RPS19 gene in 40 unrelated individuals with Diamond-Blackfan anemia (105650), Draptchinskaia et al. (1999) found 9 different mutations in 10 probands. Six of the patients with mutations had a family history of the disorder. No mutations were found in the 5-prime untranslated region or in the sequence encoding the 5 translated exons in 30 other probands. All patients with mutations were heterozygous for the alterations and no additional sequence variations in the protein-coding region of the gene were found.